Catalytic Spectrofluorimetric Determination of Folacin in Microemulsion Medium

A new catalytic kinetic spectrofluorimetric method has been developed for the indirect determination of folacin in microemulsion medium,based on the catalytic effect of palladium(Ⅱ) on the oxidation reaction between folacin and potassium periodate.The optimum condition and the kinetic parameters of this reaction are studied.The linear range is 6.0×10-5~1.0×10-6 mol/L.The detection limit is 1.0×10-7 mol/L,the relative standard deviation is less than 2.20%,and the recovery is 97%~105%.CTMAB microemulsion media is introduced into folacin determination,and it has markedly improved the sensitivity of the system.The method possesses high sensitivity as well as high selectivity.It has been used to determine folacin in tablets samples with satisfactory results.