Biochemical changes in anthers of "Dong A" genetic male sterile lines of cotton

Carbohydrates, free amino acids, IAA, GA_3 and ABA contents in anthers of different stages of Dong A recessive genetic male sterile lines (Kang A_1, MA)were measured. Less starch accumulation and abnormal contents of 4 free amino acids were detected in sterile anchers. The sterile anthers had higher contents of aspartic acid and lower contents of proline , agrinine and phenylalanine than that of fertile anthers. Markerly higher ABA content, lower IAA and GA_3 contents than the fertile anthers were also detected in all four anther development stages. Abnormal contents of Carbohydrates ,free amino acids and Phytohomones may be associated with the pollen abortion.