2013•Sichuan dongwuRequires access

PCR-RFLP Analysis of Polymorphisms of Lactate Dehydrogenase B in Maiwa Yak

Zheng Yu-cai

Abstract

In order to study the polymorphism of Ldhb gene which encodes H-subunit of lactate dehydrogenase-1(LDH1) in yak(Bos grunniens) at molecular level,the genomic DNA was extracted from 79 Maiwa yaks with 5～7 tandem repeats,and PCR-RFLP method was applied to analyze the genetic polymorphism of Ldhb,meanwhile,myoglobin content was determined in longissimus dorsi muscle.PCR-RFLP method was used to discover the mutation of G896A of yak Ldhb,and two genotypes(Ldhb-AA and Ldhb-AG) were found in 79 Maiwa yaks,genotype frequency of Ldhb-AG was 16.46%.Furthermore,these samples showed faster migration rate of lactate dehydrogenase-1 than the samples carrying Ldhb-AA during electrophoresis.Ldhb-GG genotype was not observed in this experiment.No significant correlation showed between Ldhb polymorphism and myoglobin content in longissimus dorsi muscles of Maiwa yaks.

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Key concepts: Genotype, Lactate dehydrogenase, Molecular biology, YAK, Myoglobin, Restriction fragment length polymorphism, Biology, Polymorphism (computer science)