2003•Chaye kexueRequires access

Genetic Diversity of Two Sexual Tea Cultivars Detected by RAPD Markers

Hongwei Yang

Abstract

RAPD markers analysis on the genome DNA of two sexual cultivars of Yunnadaye and Nankunshan maoyecha were studied by amplifying with 36 arbitrary 10-mer primers in order to defect the genetic diversity and relationship. Among the two sexual tea cultivars, 4382 loci were amplified, 109.55 loci per cultivars. In the total 315 DNA bands, 294 bands were polymorphic, the genetic diversity was 93.33%. It has been proved that Yunnadaye possess much higher genetic diversity than Nankunshan maoyecha on DNA molecular level. According to the cluster analysis, the two sexual cultivars might be divided into two groups by UPGMA method. 20 plants of Yunnadaye were classified as one group and 20 plants of Nankunshan maoyecha were classified as another group.

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Key concepts: RAPD, UPGMA, Genetic diversity, Biology, Cultivar, Genetics, Genetic marker, Molecular marker