Effects of magnetic field on expression of glial fibrillary acidic protein after cerebral ischemia/reperfusion in rats

To explore the effect of magnetic field on the expression of glial fibrillary acidic protein(GFAP) after cerebral ischemia/reperfusion in rats.Method:The model of cerebral ischemia/reperfusion in rats was induced with intraluminal middle cerebral artery occlusion(MCAO) with nylon monofilament suture. Twenty-four male adult SD rats were randomly divided into model group(n=12) and magnetic therapy group(n=12), then each group was randomly divided into3 d and 7d subgroups, with 6 rats in each subgroup.The magnetic therapy group was treated with pulsed magnetic field(0—10.5m T, 50 Hz, 20min/times, 1 times/d) after operation, the model group was treated in the same way but turning off the power. Both groups were evaluated with the modified neurological severity score(m NSS) at 3d and 7d after treatment(before being executed), and brain tissue slices were immunohistochemically stained to observe the expression of GFAP around the ischemic sites.Result:Compared to the model group, in 7d magnetic therapy subgroup the m NSS scores decreased significantly(P0.05), and the number of GFAP increased significantly(P0.05).Conclusion:Pulsed magnetic field can increase the expression of GFAP after cerebral ischemia/reperfusion in rats,and promote the tissue repair.