Activity of Yiye Anti-influenza Capsule Against Influenza A /H1N1 Virus in vitro and in vivo

Objective: To investigate the effect of Yiye Anti-influenza capsule( YYAFC) on the resistance to influenza A /H1N1 virus. Method: ICR mice were randomly divided into control group( 0. 5% CMC,20 mL·kg- 1),ribavirin( 0. 07 g·kg- 1),influenza virus particles group( 4.24 g·kg- 1) and YYAFC group( 1. 5,0. 75,0.38 g·kg- 1). The pneumonic model of mice were infected by influenza A( H1N1) with nasal drops,mice were administrated after infection virus in treatment andprecautiongroups. The lung index,death rate,death protection rate,the average survival period and life prolongation were calculated; cytopathic effect( CPE)was used to evaluate the anti-influenza virus( H1N1) effect of YYAFC in vitro. Result: Compared to normal group,mice were infected with the A /H1N1 influenza virus( FM1 and PR8),the lung indexin treatment and precaution groupswassignificantly increased( P 0. 01),death rate was 90% and 90% in treatment group and85% and 55% in precautiongroup,respectively,the average survival period was 9. 20 days and 9. 25 days in treatment griups and 9. 35 days and 11. 60 days in precaution group. In the treatment group of high and medium dosage YYAFC,the lung index decreased significantly,while the life span of mice was prolonged. In the precaution group of high and medium dosage YYAFC,the life span of mice was prolongedsignificantly. The IC50 of the sample inhibiting CPE caused by FM1 in vitro with doses of 0. 89 g·L- 1and the TI was 6. 75. Conclusion:YYAFC showed inhibitory effect on influenza A virus FM1 stain in vivo and in vitro.