The inhibition of BIU 87 cell proliferation by liposome conjugated antisense oligonucleotides targeting the mRNA encoding proliferating cell nuclear antigen

Objective To evaluate the inhibition effect of liposome conjugated antisense oligonucleotides on the BIU 87 proliferation. Methods The human bladder cancer cell line BIU 87 was treated with antisense oligodeoxynucleotides targeting the mRNA encoding proliferating cell nuclear antigen (PCNA) with lipofectin.The inhibition of proliferation was studied with cell growth curves and MTT method.The expression of PCNA was detected immunohistochemically. Results The cell proliferation was inhibited effectively by antisense oligodeoxynucleotides with lipofectin ( P 0.05).The protein expression of PCNA was inhibited completely at 12 hours and effectively at 24 to 36 hours ( P 0.01).Lipofectin could enhance the effect by about 50 times.The sense oligodeoxynucleotides showed no such effect ( P 0.01). Conclusions Antisense oligodeoxynucleotides conjugated with lipofectin might be a hopeful method to inhibit the proliferation of bladder cancer cell by inhibiting the expression of PCNA.Further investigations are needed in order to use this method in bladder cancer therapy.