Extraction and Purification of Total Iridoid Glycosidesfrom Herba Lamiophlomis Rotata in Industrialization

Objective:The macroporous adsorptive resins chromatotraphic column combining with polyamide chromatographic column was used to separate the flavonoides,iridoid glycosides and maximum polarity ingredients in industrialization.Method: The flavonoides,iridoid glycosides and maximum polarity ingredients were separated and enriched by CCPP serial laminar analysis equipment,the content of total iridoid glycosides was assayed by the first derivative spectrophotometry;the content of shanzhiside methylester and 8-O-acetyl shanzhiside methylester was determined by HPLC and the change of total iridoid glycosides was investigated in different elution requirement.Result: 70% alcohol was used as dissolvent.Using the 'introduction from inferior surface' sample introduction mode and 'introduction from superior surface' eluting mode in the macroporous adsorptive resins,the yield was 20.80%,transfusion ratio for shanzhiside methylester and 8-O-acetyl shanzhiside methylester was 70.77% 73.36% and 68.74% respectively.Conclusion: This production technology may transform experimental achievement to industrial production successfully.