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2011•Journal of Yichun CollegeRequires access

Screening of a Lipase Producing Stain

Yu Xie

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Abstract

Objective: Isolating lipase producing bacteria from the soil and determination of the optimum temperature and pH for enzyme activity.Methods: After enriching culture,primary screening and secondary screening,The lipase producing bacterium was isolated and its enzyme activity was assaied.Results: Successful isolated a lipase producing strain and measured its enzyme activity at different temperatures and several pH.Conclusion: 30°C,pH6.5 is the optimum conditions for the production of lipase.

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Objective: Isolating lipase producing bacteria from the soil and determination of the optimum temperature and pH for enzyme activity.Methods: After enriching culture,primary screening and secondary screening,The lipase producing bacterium was isolated and its enzyme activity was assaied.Results: Successful isolated a lipase producing strain and measured its enzyme activity at different temperatures and several pH.Conclusion: 30°C,pH6.5 is the optimum conditions for the production of lipase.

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Available abstract

Objective: Isolating lipase producing bacteria from the soil and determination of the optimum temperature and pH for enzyme activity.Methods: After enriching culture,primary screening and secondary screening,The lipase producing bacterium was isolated and its enzyme activity was assaied.Results: Successful isolated a lipase producing strain and measured its enzyme activity at different temperatures and several pH.Conclusion: 30°C,pH6.5 is the optimum conditions for the production of lipase.

Key concepts: Lipase, Enzyme, Enzyme assay, Bacteria, Strain (injury), Chemistry, Triacylglycerol lipase, Biochemistry

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