Phenotypic and DNA Mutagenic Effect by Gamma Irradiation on Purple Potato(Solanum tuberosum L.)

In order to increase the mutation frequency and obtain new mutation genetic resources, budding tubers of Hemeiren,one variety of purple potato(Solanum tuberosum L.),were treated with 60Coγirradiation at the different doses.The mutagenic effects of VMl plants were studied by phenotypic observation and molecular marker detection.The identified results of phenotypic variation showed that the irradiation dose was negatively correlated with the rate of seedling emergence.The half lethal dose of this variety was 70Gy,and the lethal dose was 80Gy.Irradiation significantly delayed emergence time,and dwarfing plantlets,decreasing number of leaves and stem diameter.Higher doses irradiation increased number of branches.Compared with control(CK),numbers of tubers per plant were not significantly different.The yields per plant decreased with the increase of irradiation dose.and the yield per plant of CK was higher than all irradiation treatments significantly.Then,DNA mutations of VM1radiation materials were detecting by using SSR molecular marker.The results showed that six polymorphic primers and ten polymorphic bands were obtained from bulk selection of ten SSR primers,and the polymorphic bands per primers were 1.7on average.Furthermore, twenty-eight amplified bands were obtained from individual detection,and eight of them were polymorphic.With 28.9%of the polymorphism rate.In conclusion,60Coγirradiation can lead to mutation of phenotype and genomic DNA on the Heimeiren variety.