Construction and primary identification of full-length cDNA library of Eisenia foetida

To obtain a full-length cDNA library of Eisenia foetida for the purpose of gene screening and functioning study,total RNA extraction method from Eisenia foetida used is TRIZOL Reagent to construct a cDNA library by the carrier of λTriplex2~(TM) using the SMART cDNA Library Construction Kit.The quality of the constructed full-length cDNA library was evaluated.The primary library showed a titer of 1.3×10~6 pfu/mL of the cDNA insets,and the amplified library has a titer of 1.0×10~8.The recombinant rates were about 99% and the average length of the cDNA inserts was about 1.1 kb.This constructed cDNA full-length library provided an essential resource for the further gene functional studies and gene discoveries of Eisenia foetida.