Detection of Extended-spectrum Beta-lactamases and Analysis of Antibiotics Resistance of Clinical Isolates from Pneumonia Patients

OBJECTIVE To analyze the productive rates of extended-spectrum beta-lactamases(ESBLs) in the clinical isolates of Gram-negative bacteria from commuity-acquired pneumonia(CAP) and hospital-acquired pneumonia(HAP) patients,and investigate their resistance to 13 kinds of antibiotics.METHODS ESBLs were detected by the double disc diffusion method and the antibiotics susceptibility test was performed by Kirby-Bauer disk diffusion method.The χ2 test was used to analyze the results.RESULTS The productive rate of ESBLs detected among CAP patients was 13.1%.The productive total rate of ESBLs detected among HAP patients was 28.36%.The former was significantly lower than the latter(χ2 test,P0.05).The resistance rates of community-acquired and hospital-acquired strains to imipenem were 2.18% and 3.54%,respectively,there was no significant difference between them(χ2 test,P0.05).Except gentamicin,there were no significant difference between the resistance of ESBLs-producing strains from CAP and from HAP to other eleven kinds of antibiotics(χ2 test,P0.05).Except imipenem and ampicillin,there was significant difference between the resistance of non-ESBLs-producing strains from CAP and from HAP to other eleven kinds of antibiotics(χ2 test,P0.05).CONCLUSIONS The productive total rate of ESBLs detected from HAP patients is significantly higher than that of from CAP patients.The supervision towards antibiotics must be strengthened to explore appropriate empirical therapy.