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2000•Chinese Journal of TraumatologyRequires access

Study on transcriptional regulation of oxidative stress on expression of surfactant protein A

FU Zu-hong

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Abstract

Objective To investigate the transcriptional regulation of oxidative stress on the expression of surfactant protein A (SPA). Methods The effect of H 2O 2 on DNA binding activity and SPA nuclear transcription activation in the SPA transcription factor TTF 1 and in regulating area of SPA gene were studied with electrophoretic mobility slow test (EMSA) and nuclear transcription test in vitro. Results H 2O 2 could inhibit the DNA binding activity and the SPA nuclear transcription activation dose-dependently in the TTF 1 and SPA gene regulating area. Conclusions Oxidative stress might be the important factor of the SPA down-expression regulation after acute lung injury.

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What this paper is about

Objective To investigate the transcriptional regulation of oxidative stress on the expression of surfactant protein A (SPA). Methods The effect of H 2O 2 on DNA binding activity and SPA nuclear transcription activation in the SPA transcription factor TTF 1 and in regulating area of SPA gene were studied with electrophoretic mobility slow test (EMSA) and nuclear transcription test in vitro. Results H 2O 2 could inhibit the DNA binding activity and the SPA nuclear transcription activation dose-dependently in the TTF 1 and SPA gene regulating area. Conclusions Oxidative stress might be the important factor of the SPA down-expression regulation after acute lung injury.

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Available abstract

Objective To investigate the transcriptional regulation of oxidative stress on the expression of surfactant protein A (SPA). Methods The effect of H 2O 2 on DNA binding activity and SPA nuclear transcription activation in the SPA transcription factor TTF 1 and in regulating area of SPA gene were studied with electrophoretic mobility slow test (EMSA) and nuclear transcription test in vitro. Results H 2O 2 could inhibit the DNA binding activity and the SPA nuclear transcription activation dose-dependently in the TTF 1 and SPA gene regulating area. Conclusions Oxidative stress might be the important factor of the SPA down-expression regulation after acute lung injury.

Key concepts: Transcription factor, Oxidative stress, Electrophoretic mobility shift assay, Transcription (linguistics), Chemistry, Cell biology, Gene expression, Nuclear protein

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