Immunohistochemically quantitative assessment of MMP-2,TIMP-1,vimentin and keratin in renal fibrosis tissues of patients with chronic allegraft nephropathy(CAN)

Objective: Renal fibrosis of patients with chronic allegraft nephropathy(CAN) usually progressed a fatal renal failure.To explore the pathogenic mechanism of renal fibrosis may provide more effective preventing and therapy means.Recently,some biomolecules playing very essential role in this lesions was reported.The histopathology and immunonisto chemistry detection were investigated in renal fibrosis specimens in present study.Methods: Renal fibrosis tissues with differnent degrees were collected from patients with CAN and 10 normal kidney specimens of non-nephropathy patients for investigation.Anti-MMP-2,Anti-TIMP-1,Anti-vimentin,and anti-Keratin,monoclonal antibodies immunostaining and an image quantitative assessment was carried out by conventional means.Results: In the early staging of renal fibrosis,positive immunostaining of MMP-2 and keratin revealed more strong and the volume increasing compared with normal renal specimmens.The degree of renal fibrosis increasing,a positive immunostaining of above two agents showed more weakly and volume declining even revealed negative.Meanwhile anti-TIMP-1 and vimentin immunostaining revealed a strong positive and the volume increasing associated with fibrosis progressing.Conclusion: The bioactivity of MMP-2 and TIMP-1 changes induced by some cytokines which produced and secreting from local vimentin-positive and related cells may play an important role in renal fibrosis of patients with CAN.