Effect of Oxaliplatin on Human Cervical Cancer HeLa Cells In Vitro

【Objective】 To study the effect of oxaliplatin on growth inhibition and caspase-3 activity on human cervical cancer HeLa cells.【Methods】 Human cervical carcinoma HeLa cells were treated with oxaliplatin.Cell growth rate was determined with MTT assay;cell morphologic changes were examined under electron microscopy.Flow cytometry and TUNEL was used to examine cell apoptosis rate.Activities of caspase-3 were determined by chromometry,and its inhibition was test by AC-DEVD-CHO.【Results】 Oxaliplatin(4-64 μmol/L) induced cytotoxicity and apoptosis to human cervical carcinoma HeLa cells in vitro is time-dependent and dose-dependent.The typical apoptotic morphological features appeared in the cells treated with oxaliplatin for 48 hours;Caspase-3 activity was up-regulated in oxaliplatin-induced apoptotic cells.The oxaliplatin induced HeLa cell apoptosis was efficiently inhibited by AC-DEVD-CHO.【Conclusion】 Oxaliplatin can inhibit HeLa cell growth of cervical carcinoma and induce apoptosis in vitro.Caspase3 activation may be involved in oxaliplatin-induced apoptosis of HeLa cells.