2013•International Journal of Digestive DiseasesRequires access

The effect of LIN28A on cell migration and invasion of hepatocellular carcinoma

Ding Chen-hon, Xi Zhang

Abstract

Objective To analyze the mRNA levels of LIN28A in liver cancer cell lines and DEN-induced rat hepatocellular carcinoma(HCC) samples,and study the function of LIN28A in tumorigenesis of HCC.Methods Examine the mRNA expression of LIN28A gene in liver cancer cells and DEN-induced rat HCC samples by real-time PCR.Detect the effect of LIN28A on cell migration and invasion of HCC.Results Real-time PCR showed that the expression of LIN28A was upregulated in twelve liver cancer cell lines and DEN-induced rat HCC.Transwell assay revealed that LIN28A overexpression significantly enhanced the cell migration and invasion of HCC.Conclusion Upregulation of LIN28A may be important for metastasis of HCC.

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Key concepts: Hepatocellular carcinoma, Downregulation and upregulation, Carcinogenesis, Liver cancer, Cancer research, Metastasis, Cell, Real-time polymerase chain reaction