2011•Hebei Nongye Daxue xuebaoRequires access
Cloning and prokaryotic expression of Bovine brucella outer membrane protein bp26 gene
Zhanxia Zhang
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Abstract
The bp26 protein gene of Bovine brucella,753 bp in length,was amplified by polymerase chain reaction(PCR) using a pair of specific primers designed according to the published DNA sequence from GenBank,and was cloned into the vetor Simple-T.Then the recombinant fragment in Simple-T was subcloned into the eukaryotic expression vector pET-28a(+).After being induced by isopropyl-b-D-thiogalactopyranoside(IPTG),the recombinant bp26 protein was expressed.The result of SDS-PAGE and Western-blot showed that the recombinant bp26 protein was 29 ku and had strong positive reactions with Bovine brucella-specific antibody.