Cloning and Expressing of Nonstructural Protein Gene NS2 of Bombyx Mori Densovisus(BmDNV-1)

The nonstructural protein gene NS2 of Bombyx mori densovisus(BmDNV-1) was cloned and expressed to study its function.NS2 gene was amplified by PCR from the infectious clone of BmDNV-1 and inserted into the prokaryotic expression vector pET-28a(+).The recombinant plasmid pET-28a-NS2 was transformed into the competent cell DH5α and the correct recombinant clone was confirmed by BamHI and HindIII digestion and sequencing.Then the plasmid pET-28a-NS2 was transferred to BL21(DE3) to express the fusion protein NS2 by induction with IPTG.The result showed that the highest expression of the fusion protein was reached after 4 hours of the induction with 1mM IPTG.The NS2 fusion protein with the size of 56.0kDa was revealed by Western blot by using antibody against His·tag which is consistent with the expected size of the fusion protein.