Effects of salvia miltiorrhiza on ischemia reperfusion injury of brain in rats

Objective:To observe the changes of brain tissue after ischemia reperfusion injury and to investigate the protective effects of salvia miltiorrhiza(SM).Methods:42 SD rats were divided into normal group(n=6),ischemia reperfusion injury group(control group,n=18) and SM group(n=18) at random.The model of ischemia reperfusion injury was made by occlusion of middle cerebral artery.SM injection was given to the rats in SM group through peritoneal injection at the dose of 5 ml/kg.The normal saline was given to the rats of the control group.The pathological changes,water ratio and cell apoptosis of these rats' brain tissue were observed after ischemia reperfusion injury in 12 h,24 h and 48 h.At the same time,the contents of MDA,glutamate(GLU) and gamma-aminobutyric(GABA) in the brain of the rats were detected.Results:Compared with the normal group,the water ratio,number of apoptotic cell,contents of MDA and GLU of the control group increased significantly and the content of GABA decreased significantly.The water ratio,number of apoptotic cell,contents of MDA and GLU of SM group were lower and GABA was higher significantly than those of the control group.Conclusion:SM can alleviate significantly the ischemia reperfusion injury of brain in rats.