Effects of Different Pretreatment Methods on Enzymatic Hydrolysis of Cotton Stalk
Pu ChunGang
Abstract
Pu ChunGang
Abstract
Effect of the chemicals dosage of dilute sulfuric acids,ulfurous acid and sulfite pretreatment and enzymatic hy-drolysis of cotton stalk was investigated.The scanning electron microscopic(SEM)analysis of untreated and treated cotton stalk was performed for comparing these methods.Under conditions of liquid ratio 1∶4t,emperature 180,℃ for 20,min,cellulase 10 U/gc,ellobiase 3.6 U/g compared to substrate,enzymatic hydrolysis conversion rate was 42.63% when 5.52% sulfuric acid with mass fraction of 98% for pretreating cotton stalk,enzymatic hydrolysis conversion rate was 81.25% when 7% sulfurous acid for pretreating cotton stalk,and enzymatic hydrolysis conversion rate was 70.06% when 0.92% sulfuric acid with mass fraction of 98% and 8% sodium bisulfite for pretreating cotton stalk.
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Effect of the chemicals dosage of dilute sulfuric acids,ulfurous acid and sulfite pretreatment and enzymatic hy-drolysis of cotton stalk was investigated.The scanning electron microscopic(SEM)analysis of untreated and treated cotton stalk was performed for comparing these methods.Under conditions of liquid ratio 1∶4t,emperature 180,℃ for 20,min,cellulase 10 U/gc,ellobiase 3.6 U/g compared to substrate,enzymatic hydrolysis conversion rate was 42.63% when 5.52% sulfuric acid with mass fraction of 98% for pretreating cotton stalk,enzymatic hydrolysis conversion rate was 81.25% when 7% sulfurous acid for pretreating cotton stalk,and enzymatic hydrolysis conversion rate was 70.06% when 0.92% sulfuric acid with mass fraction of 98% and 8% sodium bisulfite for pretreating cotton stalk.
Key concepts: Stalk, Sulfuric acid, Chemistry, Cellulase, Hydrolysis, Enzymatic hydrolysis, Nuclear chemistry, Mass fraction