2011•Zhongguo weishengtaixue zazhiRequires access
Establishment of two PC-12 cell models of persistent infection by two respective types of strains of Borna disease virus
Peng Ping Xie
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Abstract
Objective Two PC-12 cell models of persistent infection by two types of strains of Borna disease virus(BDV) were respectively established to provide a tool for studying pathogenesis of BDV infection and comparing pathogenesis feature between these two types of BDV strains.Method PC-12 cells were infected respectively with BDV strain Hu and strain V,and subcultivated for passages.BDV nucleic acid and expression of BDV protein were then detected by Real time FQ RT-PCR,Western blot and indirect immunofluorescence.Result BDV nucleic acid and protein were both dectectable in the two groups of PC-12 cells after six passages.Conclusion BDV strain Hu and strain V can replicate and express in PC-12 cell.The two PC-12 cell models of persistent infection by two respective types of strains of Borna Disease Virus(BDV) were successfully established.