2008•Zhongguo yiyuan ganranxue zazhiRequires access

Neisseria gonorrhoeae in Male Urinary Secretion by Four Methods:A Comparison of Their Results

Xiangrong Luo

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Abstract

OBJECTIVE To analyze and compare the results by four methods which were used to examine Neisseria gonorrhoeae in male urinary secretion,and provide a simple and applicable method.METHODS Totally 226 samples were examined by Wright′s and Gram′s staining for N.gonorrhoeae,some of which were examined by PCR and cultivation.RESULTS The positive rates of Wright′s and Gram′s staining were 71.68% and 73.89%,respectively.There was no statistical difference(P0.05).Compared to cultivation whose positive rate was 33.33%,the rates were obvious higher(P0.05),but they were lower than PCR whose positive rate was 85.71%(P0.05).CONCLUSIONS For the examination of N.gonorrhoeae in male urinary secretion,the positive rate of PCR is the highest,and the positive rate of staining is better,in which Wright′s staining is more convenient,quicker and cheaper.The positive rate of cultivation is the lowest,and it isn′t applied to general examination.

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OBJECTIVE To analyze and compare the results by four methods which were used to examine Neisseria gonorrhoeae in male urinary secretion,and provide a simple and applicable method.METHODS Totally 226 samples were examined by Wright′s and Gram′s staining for N.gonorrhoeae,some of which were examined by PCR and cultivation.RESULTS The positive rates of Wright′s and Gram′s staining were 71.68% and 73.89%,respectively.There was no statistical difference(P0.05).Compared to cultivation whose positive rate was 33.33%,the rates were obvious higher(P0.05),but they were lower than PCR whose positive rate was 85.71%(P0.05).CONCLUSIONS For the examination of N.gonorrhoeae in male urinary secretion,the positive rate of PCR is the highest,and the positive rate of staining is better,in which Wright′s staining is more convenient,quicker and cheaper.The positive rate of cultivation is the lowest,and it isn′t applied to general examination.

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Available abstract

OBJECTIVE To analyze and compare the results by four methods which were used to examine Neisseria gonorrhoeae in male urinary secretion,and provide a simple and applicable method.METHODS Totally 226 samples were examined by Wright′s and Gram′s staining for N.gonorrhoeae,some of which were examined by PCR and cultivation.RESULTS The positive rates of Wright′s and Gram′s staining were 71.68% and 73.89%,respectively.There was no statistical difference(P0.05).Compared to cultivation whose positive rate was 33.33%,the rates were obvious higher(P0.05),but they were lower than PCR whose positive rate was 85.71%(P0.05).CONCLUSIONS For the examination of N.gonorrhoeae in male urinary secretion,the positive rate of PCR is the highest,and the positive rate of staining is better,in which Wright′s staining is more convenient,quicker and cheaper.The positive rate of cultivation is the lowest,and it isn′t applied to general examination.

Key concepts: Neisseria gonorrhoeae, Staining, Gram staining, Microbiology, Biology, Internal medicine, Medicine, Antibiotics

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