Influences of Tangshenqi on Diabetic Nephropathy in Rats Induced by STZ Injection

Objective: To investigate the protective effects of Tangshenqi on diabetic nephropathy( DN)in rats. Method: The DN model was built by intravenous injection of streptozotocin( STZ) once in sprague dawley( SD) rats. The model rats were randomly divided into six groups: model control group,high-dosage Tangshenqi group( crude drug,28. 0 g·kg- 1),middle-dosage Tangshenqi group( crude drug,14. 0 g·kg- 1),low-dosage Tangshenqi group( crude drug,7. 0 g·kg- 1),Tangmaikang granule group,and gliquidone + captopril group. The rats were continuously administrated for 12 weeks,in the same time,the general situations in rats were observed,and body weight,food intake,water intake,urine index( urine sugar and urine protein),blood index( fasting glucose,creatinine,urea,glycosylated hemoglobin) were regularly tested. After the last administration,kidney weight,kidney index,and area of glomerular positive region by PAS staining were measured,while the examination of kidney histopathology was carried out. Result: Tangshenqi could reduce blood glucose in DN rats,regulate abnormal glucose metabolism,and promote the transformation of hepatic glycogen. It could also reduce uriinary volume,as well as the content of urine sugar and urine protein,and increase glomerular filtration rate.Moreover it could inhibit kidney hypertrophy,reduce incidence of kidney disease,as well as improve and repair the degree of lesions. Conclusion: Tangshenqi could have a good protective effect on diabetic nephropathy( DN)in rats.