Effects of LY294002 on bFGF-induced proliferation of lens epithelial cells in rabbit

Background The primary mechanism of posterior capsular opacification(PCO) is the proliferation and migration of lens epithelial cells(LECs) because cytokines stimulate residual LECs following the destroy of blood-aqueous humor.To arrest the proliferation of LECs is very important for the prevention of PCO.Researches showed that LY294002 can inhibit the proliferation of tumor cells.But its effects on LECs is unclear.ObjectiveThe aim of this study was to investigate the proliferation inhibition effects of LY294002 on rabbit lens epithelial cells induced by basic fibroblast growth factor,bFGF(bFGF).MethodsThe LECs were isolated from rabbits and primarily culture and passaged.10-8,10-7,10-6,10-5,10-4 mol/L of LY294002 were added into the medium respectively for 48 hours in various experimental groups,and 10-8,10-7,10-6,10-5 mol/L,10-4 mol/L of LY294002+bFGF(10 mg/L) were added for 48 hours in combination drugs groups,and bFGF(10 mg/L) was used for 48 hours to induce the proliferation of LECs in positive control group.LECs was cultured in free-serum DMEM as blank control group.The absorbency(A value) was detected by methyl thiazolyl tetrazolium(MTT) for the assessment of inhibiting rate of LECs in different groups,and percentage of LECs in different cell cycles was tested and analyzed with flow cytometry.ResultsThe A value was elevated in bFGF group in comparison with control group.In 48 hours after LY294002 was added,the A values were gradually and significantly declined with the increase of LY294002 concentration(P0.01),and more obvious descend in A values was seen in bFGF+LY294002 groups compared with only LY294002 groups(P0.01).The inhibiting rate of LY294002 on LECs showed the same tendency.The percentage of LECs in G0/G1 phase was gradually increased(P0.01),and that in S phase and G2/M phase were significantly decreased with the rise of LY294002 concentration(P0.01).ConclusionLY294002 can effectively inhibit the proliferation of LECs of rabbit in vitro at a dose-dependent manner.This result offers a basis for the clinical treatment of PCO.