Expression of HSP70 and drug-resistance genes in non-small-cell lung cancer A549 cells treated by β-elemene with hyperthermia and cisplatin

Objective To investigate the effect on expression of HSP70,MDR1 and Bax/Bcl-2 in A549 cells treated by β-elemene with hyperthermia and cisplatin.Methods Treated by β-elemene,hyperthermia and cisplatin,RT-PCR and Western Blots were applied to detect mRNA transcription and protein expression of HSP70 and MDR1,while Bax/Bcl-2 mRNA detected by RT-PCR.Results No significant difference was observed in HSP70 mRNA transcription,but compared with control at 37℃.HSP70 protein expressions were mostly up-regulated and there was statistical significance(t=7.48～55.76,P0.05).MDR1 mRNA were mostly down-regulated,but after treatment with cisplatin 4 μg/ml and 15 μg/ml elemene in 42℃ it was up-regulated(compared with 37℃ control,or 4 μg/ml cispaltin and 15 μg/ml elemene in 42℃,t=93.38,7.99,P0.05).Bcl-2 mRNA transcriptions were mostly down-regulated and significant differences were observed(t=4.41,3.97,5.11,3.43,4.88,4.52,P0.05)compared with control,while Bax mRNA with no significant differences.Conclusions Inhibition of MDR1 and Bcl-2 result cell apoptosis when treated after high concentration elemene and cisplatin at 37℃,but after treated with low concentration elemene and cisplatin at 42℃ the cell apoptosis mainly owed to the lower expression of Bcl-2 gene.