Construction of Anti-Apoptosis CHO/dhfr-Cell Line by Overexpressing Integrted Bcl-2 Gene

To construct a new anti-apoptosis CHO/dhfr-cell line by overexpessing integrated human Bcl-2 gene in the genome.Human Bcl-2 gene was integrated into the genome of CHO/dhfr-cell by FIp-InTM recombination system.The expession of Bcl-2 protein was detected by western-blot.The level of cell apoptosis was detected by FACS and DNA ladder.Cell viability and viable cell counts were preformed with trypan blue exclusion method.Recombination cell line CHO-bcl-2 that could overespress Bcl-2 protein was constructed,the apoptosis of CHO-Bcl-2 was considerably supressed and viability of cells were improved under conditions of serum withdrawal or treatment with ammonium chloride.