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Melatonin Induced Apoptosis of H22 Cells by Increasing Expression of p53 and fas

She Mei

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Abstract

Objective:To investigate the mechanism of apoptosis effect on H22 hepatoma cells induced by melatonin(MLT).Methods:Apoptosis effect of MLT on H22 cells was studied by acridine orange(AO) staining,lactate dehydroge-nase(LDH) assay and flow cytometry(FCM).Comparing the levels of p53 mRNA and Fas mRNA between cells treated with and without MLT by RT-PCR.Results:The nuclear shrinking and fragments were obvious after AO staining.LDH activity increased after MLT treatment,and a higher rate of apoptosis was observed by FCM.The expressions of p53 and Fas gene was stimulated by MLT(P0.01).Conclusion:MLT induced apotosis of the H22 cells dependent on p53 and Fas.

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What this paper is about

Objective:To investigate the mechanism of apoptosis effect on H22 hepatoma cells induced by melatonin(MLT).Methods:Apoptosis effect of MLT on H22 cells was studied by acridine orange(AO) staining,lactate dehydroge-nase(LDH) assay and flow cytometry(FCM).Comparing the levels of p53 mRNA and Fas mRNA between cells treated with and without MLT by RT-PCR.Results:The nuclear shrinking and fragments were obvious after AO staining.LDH activity increased after MLT treatment,and a higher rate of apoptosis was observed by FCM.The expressions of p53 and Fas gene was stimulated by MLT(P0.01).Conclusion:MLT induced apotosis of the H22 cells dependent on p53 and Fas.

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Available abstract

Objective:To investigate the mechanism of apoptosis effect on H22 hepatoma cells induced by melatonin(MLT).Methods:Apoptosis effect of MLT on H22 cells was studied by acridine orange(AO) staining,lactate dehydroge-nase(LDH) assay and flow cytometry(FCM).Comparing the levels of p53 mRNA and Fas mRNA between cells treated with and without MLT by RT-PCR.Results:The nuclear shrinking and fragments were obvious after AO staining.LDH activity increased after MLT treatment,and a higher rate of apoptosis was observed by FCM.The expressions of p53 and Fas gene was stimulated by MLT(P0.01).Conclusion:MLT induced apotosis of the H22 cells dependent on p53 and Fas.

Key concepts: Apoptosis, Acridine orange, Flow cytometry, Molecular biology, Melatonin, Biology, Staining, Biochemistry

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Melatonin Induced Apoptosis of H22 Cells by Increasing Expression of p53 and fas — Research Paper | ScholarLens