The Overexpression and Related Study of p53、Ki-67、p16 and Cyclin D1 in Epithelial Dysplasia from Oral Mucosa

Objective To study the relationship between the malignant transformation of oral epithelial dysplasia and the multistep gene mutations and interaction. Methods The expression p53, Ki-67, p16 and cyclin D1 was determined in the 76 cases of oral epithelial dysplasia and 84 normal persons by immunihistochemistry, and a follow -up study of three years was made. Results The overexpression of p53 and Ki-67 in the cases of oral epithelial dysplasia was significantly higher than that in the nomal oral mucosa(P0.05), and there was corelation between the malignant trans formation and mutation of both p53 and Ki-67 in the cases of oral epithelial dysplasia. There were interruption of balance between p16 and cyclin D (the overexpression of cyclin D and the underexpression of p16) in oral epithelial dysplasia cases. Conclusion The malignant transformation of oral epithelial dysplasia cases is the results of multistep gene mutations and interaction. the overexpression of p53 and Ki-67 and the interruption of balance between p16 and cyclinD may play a important role in these processes.