ScholarLens Back to search
2009•Lishizhen Medicine and Materia Medica ResearchRequires access

Effects of Danqi Mixture on the Expression of Bone Morphogenetic Protein-7 and its Receptor in the Renal Tubules of Diabetic Rats

Guozhong Zhang

Open publisher page 0 citations

Abstract

Objective To investigate the effects of Dan qi mixture and enalapril on the expression of bone morphogenetic protein-7(BMP-7) and its receptor ALK-3 in the renal tubules of diabetic rats.Methods SD rats were randomly divided into normal control group(group A),diabetic group(group B),diabetic Danqi-treated group(group C),diabetic enalapril-treated group(group D).The diabetic rat model was made with streptozotocin.After 12 weeks,blood glucose,triglyceride,cholesterol and creatinine,urine protein were analyzed with biochemical methods.Kidney index was analyzed as well.The changes of pathomorphology were examined in PAS staining sections.Immunohistochemistry was employed to detect the expression of BMP-7 and FN protein in renal tubules of all groups.The mRNA levels of BMP-7 and its receptor ALK-3 in renal cortex were examined by RT-PCR.Results ①Compared with group A,the levels of blood glucose,triglyceride,cholesterol and creatinine,urine protein and kidney index were increased remarkably in group B.In group C and D,parameters above-mentioned except blood glucose were notably cut down.② The expression of BMP-7 protein was high and FN protein was slight by immunohistochemistry in group A.On the other hand,the protein of BMP-7 was decreased remarkably in group B,and the protein of FN was increased.In group C and D,the expression of BMP-7 protein was up-regulated notably,but the protein of FN was decreased.③ Compared with group A,the expression of BMP-7 mRNA and its receptor ALK-3 decreased remarkably by RT-PCR in group B.In group C and D,the expression of the BMP-7 and ALK-3 mRNA was up-regulated notably compared to group B,and the expression of ALK-3 mRNA in group D was even higher than that in group A.Conclusion The Danqi mixture and enalapril can promote the expression of endogenous BMP-7 and its receptor ALK-3 in the renal tubule cells,and therefore reducts the deposition of FN and protects the kidney of diabetic rats.

About this research paper

What this paper is about

Objective To investigate the effects of Dan qi mixture and enalapril on the expression of bone morphogenetic protein-7(BMP-7) and its receptor ALK-3 in the renal tubules of diabetic rats.Methods SD rats were randomly divided into normal control group(group A),diabetic group(group B),diabetic Danqi-treated group(group C),diabetic enalapril-treated group(group D).The diabetic rat model was made with streptozotocin.After 12 weeks,blood glucose,triglyceride,cholesterol and creatinine,urine protein were analyzed with biochemical methods.Kidney index was analyzed as well.The changes of pathomorphology were examined in PAS staining sections.Immunohistochemistry was employed to detect the expression of BMP-7 and FN protein in renal tubules of all groups.The mRNA levels of BMP-7 and its receptor ALK-3 in renal cortex were examined by RT-PCR.Results ①Compared with group A,the levels of blood glucose,triglyceride,cholesterol and creatinine,urine protein and kidney index were increased remarkably in group B.In group C and D,parameters above-mentioned except blood glucose were notably cut down.② The expression of BMP-7 protein was high and FN protein was slight by immunohistochemistry in group A.On the other hand,the protein of BMP-7 was decreased remarkably in group B,and the protein of FN was increased.In group C and D,the expression of BMP-7 protein was up-regulated notably,but the protein of FN was decreased.③ Compared with group A,the expression of BMP-7 mRNA and its receptor ALK-3 decreased remarkably by RT-PCR in group B.In group C and D,the expression of the BMP-7 and ALK-3 mRNA was up-regulated notably compared to group B,and the expression of ALK-3 mRNA in group D was even higher than that in group A.Conclusion The Danqi mixture and enalapril can promote the expression of endogenous BMP-7 and its receptor ALK-3 in the renal tubule cells,and therefore reducts the deposition of FN and protects the kidney of diabetic rats.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To investigate the effects of Dan qi mixture and enalapril on the expression of bone morphogenetic protein-7(BMP-7) and its receptor ALK-3 in the renal tubules of diabetic rats.Methods SD rats were randomly divided into normal control group(group A),diabetic group(group B),diabetic Danqi-treated group(group C),diabetic enalapril-treated group(group D).The diabetic rat model was made with streptozotocin.After 12 weeks,blood glucose,triglyceride,cholesterol and creatinine,urine protein were analyzed with biochemical methods.Kidney index was analyzed as well.The changes of pathomorphology were examined in PAS staining sections.Immunohistochemistry was employed to detect the expression of BMP-7 and FN protein in renal tubules of all groups.The mRNA levels of BMP-7 and its receptor ALK-3 in renal cortex were examined by RT-PCR.Results ①Compared with group A,the levels of blood glucose,triglyceride,cholesterol and creatinine,urine protein and kidney index were increased remarkably in group B.In group C and D,parameters above-mentioned except blood glucose were notably cut down.② The expression of BMP-7 protein was high and FN protein was slight by immunohistochemistry in group A.On the other hand,the protein of BMP-7 was decreased remarkably in group B,and the protein of FN was increased.In group C and D,the expression of BMP-7 protein was up-regulated notably,but the protein of FN was decreased.③ Compared with group A,the expression of BMP-7 mRNA and its receptor ALK-3 decreased remarkably by RT-PCR in group B.In group C and D,the expression of the BMP-7 and ALK-3 mRNA was up-regulated notably compared to group B,and the expression of ALK-3 mRNA in group D was even higher than that in group A.Conclusion The Danqi mixture and enalapril can promote the expression of endogenous BMP-7 and its receptor ALK-3 in the renal tubule cells,and therefore reducts the deposition of FN and protects the kidney of diabetic rats.

Key concepts: Endocrinology, Internal medicine, Bone morphogenetic protein 7, Kidney, Triglyceride, Creatinine, Immunohistochemistry, Streptozotocin

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effects of Danqi Mixture on the Expression of Bone Morphogenetic Protein-7 and its Receptor in the Renal Tubules of Diabetic Rats — Research Paper | ScholarLens