Effect of HPV16 E7 specific-siRNA expression on biological activity of cervical cancer CaSki cells

Objective To investigate a new potential therapy to achieve targeted inhibition of HPV16 E7 oncogene in tumor cells using small interfering RNAs(siRNA).Methods The HPV16 E7 siRNA and scramble control expression plasmids were structured and transfected into CaSki cells by liposome.The stable cell lines of HPV16 E7 siRNA were established by Hygromycin screening.The expression levels of HPV16 E7 mRNA and protein were detected by RT-PCR,immunofluorescence and flowcytometry.The cell cycle and cell proliferation were measured with flowcytometry and MTT method.Meanwhile the cisplatin chemotherapy sensitivity was observed in the cells.Results Plasmids HPV16 E7 siRNA and scramble control were constructed and confirmed by sequencing.HPV16 E7 mRNA and protein expressions were significantly decreased in the HPV16-E7Si cells compared with the CaSki-Si control cells.The cell growth rate of HPV16-E7Si cells was significantly inhibited.Flowcytometry analysis revealed a significant increase in apoptosis.In addition,after cisplatin(DDP) treatment for 48 hours the ratio of G0~1 phase of HPV16-E7Si cells was increased compared with control cells.Conclusion HPV16 E7 siRNA could successfully suppress CaSki cell growth and induce the cell apoptosis,and increases sensitivity of the cisplatin chemotherapy.