Micrometastasis detection in breast cancer of patients by biological markers CK19,hMAM and SBEM

OBJECTIVE:To disscus the clinical application of biological markers small breast epithelial mucin,human mammaglobin,and cytokeratin19by detecting circulating tumor cells and lymph node micrometastasis in breast cancer. METHODS:The peripheral blood and tissue samples were collected in diagnosed patients of breast cancer from April 13, 2012to October 10,2012,Department of Breast Surgery,Tumor Hospital Affiliated to Guangxi Medical University. SBEM,hMAM,and CK19mRNA were dected by nested PCR in peripheral blood of 47cases of breast cancer and 45cases of benign breast tumor.Semi-quantitative PCR method was used to detected the mRNA express of 10cases of breast cancer tissue,10cases of paracancerous tissue and 10cases of benign breast tumor tissue.The metastasis of 109lymph nodes in 27cases of breast cancer patients were detected using immunohistochemistry technique.RESULTS:SBEM expression in peripheral blood of breast cancer and benign breast tumor patients were 82.98%(39/47)and 62.22%(28/45),respectively(χ 2=5.005,P=0.021);hMAM expression in peripheral blood of breast cancer and benign breast tumor patients were 65.96%(31/47)and 42.22%(19/45),respectively(χ 2=5.220,P=0.022),the difference was statiscally significant. CK19expression in peripheral blood of breast cancer and benign breast tumor patients were 89.36%(42/47)and 80.00%(36/45),respectively(χ 2=1.562,P=0.211),the difference was no statisca significace.Fifty-two lymph nodes metastasis by HE staining,there had 5lymph nodes micometastasis were found by CK19in 57negative lymph nodes diagnosed by routine pathology.hMAM and SBEM expression in metastatic lymph nodes was weaker than that of CK19and the positive cells were not uniform.CONCLUSIONS:SBEM,hMAM,and CK19as biomarkers for breast cancer micrometastases in peripheral blood have high sensitivity and low specificity.Joint detection could improve the specificity and it has some clinical significance for the early detection of breast cancer micrometastases.CK19may be used to detect axillary lymph node micrometastases.