Relationship between irradiation dose and expressions of bcl-2,bax genes in apoptosis process of pancreatic carcinoma cells in vitro

Objective To explore the relationship between irradition dose and expressions of bcl-2,bax genes in apoptosis process of pancreatic carcinoma cells. Methods Apoptosis was detected with PI and AnnexinⅤ/PI methods and bcl-2,bax proteins in Panc-1 cells after gamma-irradiation were determined with flow cytometry. Results The results showed that the apoptotic rate increased along with the increase of dose and time within a certain range (≤15 Gy,≤24 h). The expression of bcl-2 was significantly lower in the irradiated cells than that in the control cells (P0.05),but that of bax was higber in the former than that in the latter (P0.05). Conclusion Gamma-radiotherapy can induce apoptosis in pancreatic carcinoma cells through down-regulation of the expression of bcl-2 and up-regulation of the the expression of bax.;