ScholarLens Back to search
2005•Zhōnghuá yàoxué zázhìRequires access

Effects of tanshinon on VSMC proliferation and the expression of pp-ERK1/2 protein in rats

Xiu Gao

Open publisher page 1 citations

Abstract

OBJECTIVE To investigate the effects of tanshinone on rat vascular smooth muscle cell(VSMC) proliferation and ERK signaling METHODS Rat dorsal aortic VSMCs were cultured by tissue sticking method MTT and protein assay were used to estimate the proliferation activity of VSMC Western blot was used to assess the protein expression of pp ERK1/2 RESULTS Tanshinone inhibited VSMC proliferation response stimulated by 5% fetal bovine serum (FBS) and serum stimulated pp ERK1/2 expression in a dose dependent fashion Inhibition rates of 2,10,50 μg·mL -1 tanshinone were (17 7±3 6)%,(26 2±4 1)% and (72 7±3 8)%( P 0 05 vs control), respectively CONCLUSION The results suggested that tanshinone down regulated the expression of pp ERK1/2, which might be the mechanism in depressing VSMC proliferation

About this research paper

What this paper is about

OBJECTIVE To investigate the effects of tanshinone on rat vascular smooth muscle cell(VSMC) proliferation and ERK signaling METHODS Rat dorsal aortic VSMCs were cultured by tissue sticking method MTT and protein assay were used to estimate the proliferation activity of VSMC Western blot was used to assess the protein expression of pp ERK1/2 RESULTS Tanshinone inhibited VSMC proliferation response stimulated by 5% fetal bovine serum (FBS) and serum stimulated pp ERK1/2 expression in a dose dependent fashion Inhibition rates of 2,10,50 μg·mL -1 tanshinone were (17 7±3 6)%,(26 2±4 1)% and (72 7±3 8)%( P 0 05 vs control), respectively CONCLUSION The results suggested that tanshinone down regulated the expression of pp ERK1/2, which might be the mechanism in depressing VSMC proliferation

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE To investigate the effects of tanshinone on rat vascular smooth muscle cell(VSMC) proliferation and ERK signaling METHODS Rat dorsal aortic VSMCs were cultured by tissue sticking method MTT and protein assay were used to estimate the proliferation activity of VSMC Western blot was used to assess the protein expression of pp ERK1/2 RESULTS Tanshinone inhibited VSMC proliferation response stimulated by 5% fetal bovine serum (FBS) and serum stimulated pp ERK1/2 expression in a dose dependent fashion Inhibition rates of 2,10,50 μg·mL -1 tanshinone were (17 7±3 6)%,(26 2±4 1)% and (72 7±3 8)%( P 0 05 vs control), respectively CONCLUSION The results suggested that tanshinone down regulated the expression of pp ERK1/2, which might be the mechanism in depressing VSMC proliferation

Key concepts: Vascular smooth muscle, Western blot, Fetal bovine serum, Smooth muscle, Cell growth, MAPK/ERK pathway, Protein expression, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effects of tanshinon on VSMC proliferation and the expression of pp-ERK1/2 protein in rats — Research Paper | ScholarLens