Study of Type-specific Oligonucleotide Probes for Microarray Detection of Human Papillomavirus

High-risk human papillomavirus (HPV) types are major causes of cervical cancer. By using bioinformatics softwares such as BLAST,Arraydesigner2.0 to analyze the whole genome sequence of 10 prevalent HPV types and design type-specific oligonucleotide microarray probes of high specificity,identical length and similar melting temperature (Tm). These type-specific oligonucleotide probes were used to prepare microarray for HPV detection. And the validity of four HPV types (HPV 6,11,16,18) probes were validated preliminarily,showing that most of the type specific oligonucleotide probes can be effectively applied to the HPV detection and typing microarray.