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2010•Zhongguo yaolixue tongbaoRequires access

Apoptosis of B16 melanoma cells induced by berberine α-hydroxy-β-decanoylethyl sulfonate

Huayi Peng

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Abstract

Aim To investigate the mechanism of induction of cell apoptosis of berberine α-hydroxy-β-decanoylethyl sulfonate(HB).Methods MTT assay was employed to determine the cytotoxicity of HB in B16 cells.Through Rhodamine 123 staining,agarose gel electrophoresis,Hoechst 33258 staining,activity of caspase-3 and caspase-8 was detected,and B16 cell apoptosis induced by HB was observed.Results HB inhibited the B16 cell proliferation in vitro.The inhibitory effect was dose and time dependent,and the IC50 was 16.59,9.29 and 6.22 mg·L-1 when B16 cell was exposed to HB for 24,48 and 72h,respectively.B16 cells treated with HB showed typical characteristics of apoptosis:decreased Rhodamine 123 fluorescence intensity,karyopyknosis and DNA Ladder.Caspase-3 and caspase-8 might be involved in this process.Conclusion HB could inhibit cell growth and induce apoptosis of B16 cells.

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What this paper is about

Aim To investigate the mechanism of induction of cell apoptosis of berberine α-hydroxy-β-decanoylethyl sulfonate(HB).Methods MTT assay was employed to determine the cytotoxicity of HB in B16 cells.Through Rhodamine 123 staining,agarose gel electrophoresis,Hoechst 33258 staining,activity of caspase-3 and caspase-8 was detected,and B16 cell apoptosis induced by HB was observed.Results HB inhibited the B16 cell proliferation in vitro.The inhibitory effect was dose and time dependent,and the IC50 was 16.59,9.29 and 6.22 mg·L-1 when B16 cell was exposed to HB for 24,48 and 72h,respectively.B16 cells treated with HB showed typical characteristics of apoptosis:decreased Rhodamine 123 fluorescence intensity,karyopyknosis and DNA Ladder.Caspase-3 and caspase-8 might be involved in this process.Conclusion HB could inhibit cell growth and induce apoptosis of B16 cells.

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Available abstract

Aim To investigate the mechanism of induction of cell apoptosis of berberine α-hydroxy-β-decanoylethyl sulfonate(HB).Methods MTT assay was employed to determine the cytotoxicity of HB in B16 cells.Through Rhodamine 123 staining,agarose gel electrophoresis,Hoechst 33258 staining,activity of caspase-3 and caspase-8 was detected,and B16 cell apoptosis induced by HB was observed.Results HB inhibited the B16 cell proliferation in vitro.The inhibitory effect was dose and time dependent,and the IC50 was 16.59,9.29 and 6.22 mg·L-1 when B16 cell was exposed to HB for 24,48 and 72h,respectively.B16 cells treated with HB showed typical characteristics of apoptosis:decreased Rhodamine 123 fluorescence intensity,karyopyknosis and DNA Ladder.Caspase-3 and caspase-8 might be involved in this process.Conclusion HB could inhibit cell growth and induce apoptosis of B16 cells.

Key concepts: Apoptosis, Molecular biology, Chemistry, Staining, Agarose gel electrophoresis, MTT assay, Cytotoxicity, Cell growth

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