Study on protective effects of lutein on acute lung injury induced by lipopolysaccharide in mice

Objective To study the protective effects of lutein against acute lung injury induced by lipopolysaccharide and its possible mechanism in mice.Methods Randomly distributed 60 male Kunming mices into control group,lipopolysaccharide-induced acute lung injury model group(ALI),dexamethasone(DXM)group(5 mg/kg)and low,middle,high dose groups of lutein(10,15,20 mg/kg).After 30d with different dosage of lutein,control group was given physiological saline,ALI model group,lutein administrated groups and DXM group were injected with lipopolysaccharide(LPS)(6.0 mg/kg)to induce ALI.Six hours after the injection,abdominal aorta blood was collected for measuring of lymphocyte subpopulations(CD+3,CD+4,CD+8),tumour factor-α(TNF-α),leckocyte interpose-8(IL-8),malondialdehyde(MDA)content,superoxide dismutase(SOD),glutathione perioxidase(GSH-Px)activities.And lung wet weight/dry weight ratio,neutrophil MPO activity of lung TNF-α,leckocyte interpose-10(IL-10)content of lung were measured.Results Treatment with different dosage of lutein could significantly decrease serum TNF-α(model group:475.29±93.12;lutein groups from low dose to high dose:390.10±81.50,374.20±80.09,340.18±84.39);IL-8(model group:124.56±20.21;lutein groups from low dose to high dose:111.13±16.30,107.33±15.39,103.39±14.36);MPO(model group:6.02±1.06;lutein groups from low dose to high dose:5.12±1.02,5.03±0.80,4.48±0.73);MDA content(model group:11.28±2.14;lutein groups from low dose to high dose:9.20±0.62,8.29±1.30,7.10±1.21).There was a reduction of IL-10 in lung(model group:60.13±20.28;lutein groups from low dose to high dose:71.23±22.39,78.28±21.27,85.18±28.15).The concentrations of SOD were(model group:74.52±24.40;lutein groups from low dose to high dose:114.30±41.50,130.53±40.23,149.19±41.77).GSH-Px activities were(model group:62.24±10.13;lutein groups from low dose to high dose:77.70±12.15,85.20±12.03,90.47±13.12).The lymphocyte subpopulations of the blood was improved.Conclusion Lutein showed protective effects on the acute lung injury induce by LPS in mice.