Effects of Aloe Polysaccharides on the Nitric Oxide Production and iNOS activity of Mice Peritoneal Macrophages

 Objective To investigate the effects of nitric oxide and inducible nitric oxide synthase (iNOS) activity of mouse peritoneal macrophages in vitro by Aloe Polysaccharides. Methods The mouse peritoneal macrophages were stimulated in 24h with 0～400mg/L concentration respectively. NO production and iNOS activity were determined by Griess and fluorometric method. Results Stimulated mouse peritoneal macrophages were with 25～400mg/L concentration respectively after 24 h, NO production and iNOS activity was increased, and there were significant differences in all groups(P0.01) except low AP concentration(25mg/L) and notable positive correlation(r=0.7523,P0.01). The result also showed that mRNA transcriptional inhibitor Actinomycin D, protein synthesis inhibitor Actidione and NOS competitive inhibitor N-monomethyl-L-arginine(L-NMMA) could effectively inhibit NO production and intracellular iNOS activity of macrophages induced by AP(P0.01). Conclusion AP can enhance intracellular iNOS gene expression, synthesis and releasing of cellular NO. NO may take part in the immunoregulation of AP. 