Quantification of the Total Saponins Content in Radix pseudostellariae

The mensuration condition( inspecting solvent extraction,solid-liquid ratio,partical size,extraction time and color conditions) of total saponins conten in Radix Pseudostellariae were preliminarily established by the single factor experiment. Then the method of preparing testing solution was optimized by the orthogonal test. The results showed that when the solid-liquid ratio of Radix Pseudostellariae powder and ethanol was 0. 1 g∶ 20 mL,the time of ultrasonic extraction was 120 min,the color conditions were 5% vanillinacetic acid and 60% sulfuric acid,the result of determination was optimum. After the tests of precision,stability,repeatability and sample recovery rate,RSD 3%,the method of determination was stable and reliable,and can be used to detecte total sapoins in Radix Pseudostellariae.