Effects of endothelial microparticles on expressions of VCAM-1 and ICAM-1 in human umbilical vein endothelial

Objective:To examine the effects of endothelial microparticles(EMPs)on the expressions of vascular cell adhesion molecule 1(VCAM-1) and intercellular adhesion molecule(ICAM-1) in human umbilical vein endothelial cells(HUVECs).Methods:HUVECs at passage 4 to 5 were incubated for 24 h with EMPs(0,1×102,1×103,1×104,1×105/ml).Quantitative real-time polymerase chain reaction and Western blotting were used to detect expressions of mRNA and protein of ICAM-1 and VCAM-1 respectively.Results:Both two detection methods showed that EMPs stimulation significantly induced the mRNA and protein expressions of VCAM-1 and ICAM-1 in HUVECs in a dose-dependent manner.Conclusion:EMPs can induce the expressions of VCAM-1 and ICAM-1 in a dose-dependent manner.These findings suggest that EMPs may have adverse effects in modulating inflammatory response in atherosclerosis.