Establishment of radioresistant glioma cell subline

Objective To study the method of inducing and building glioma subline with radiation resistance,and then to investigate the potential mechanism of the radioresistance in glioma cell. Methods The subject was human U251 glioma cell line,cultivating U251 cells in vitro, simulating the clinical radiotherapy,the U251 cells were repeatedly given individual dose with60Co resource to induce radiation resistance U251( RR-U251). After 5 Gy radiotherapy,the intracellular ultrastructure of the strains were observed by microscopy,MTT assay was examined the cell proliferation,the cell apoptosis rate was examined by flow cytometry( FCM) and the cell radiosensitivity were assayed by colony formation assay. Results The proliferation increased 11. 5%, the apoptosis rate decreased 69. 6% compared with the U251. Cell migration and invasion ability were increased 50% and 87. 5% respectively. Cell clone analysis demonstrated that RR-U251 had greater plating efficiency( PE),after 5 Gy irradiation increased by 88. 5%. Conclusion The method of radiating cells is viable to establish radioresistant glioma cell line-RR-U251.