Safety of Blocking the Hepatic Artery in Rats with Obstructive Jaundice and Its Influence on Liver Cell Apoptosis

Objective To investigate the safety and mechanism of blocking or cutting hepatic artery in obstructive jaundice rats with biliary enteric drainage,by comparing serum bilirubin,pathological changes and changes of liver cell apotposis in animal models of obstructive jaundice rats cutting or non-cutting hepatic artery during biliary enteric drainage.Method 48 male SD rats were randomly divided into groups A,B,C and D,and made into obstructive jaundice model by ligating common bile duct,enteric drainage was adopted and hepatic artery was cutted in groups C and D 7 days later.Rats in groups A and C were scarificed 7days later while rats in groups B and D were killed 14 days later.Speciments of blood and liver tissue were collected to test.Results One rabbit died in group A,the time of death was the third day after biliary enteric drainage,the rate of death was 8.33%.Two rabbits died in group D,the time of death was the 10th and 12th day after biliary enteric drainage,the rate of death was 16.67%.The difference of death rate between two groups had no statistical significance(P0.05).The TBIL and AI in group A were higher than those in group C,while those in group D were also higher than those in group B,and the difference had statistical significance(P0.05).The morphological changes of liver pathology(such as liver fibrosis,liver cell necrosis and inflammatory cell infiltration) in hepatic artery ligation group were more serious than those in non-ligation group.Conclusions It is unsafe to cut hepatic artery,and it has a great impact on apoptosis of liver cells when biliary drainage was underwent in obstructive jaundice rats.