The Comparatiue Effect of JGB on the Bone Histomorphometry and Bone Quality Parameter in Ovariectomized Rats

ObjectiveTo investigate the changes of the compound of Astragalus membranaceus and Stanozolol(JGB) on the bone histomorphometry and bone quality parameter such as Calcium,Magnesium,Phosphorus,hydroxyproline and bone weight in ovariectomized(OVX) osteoprosis rats,and analyse the mutuality between the changes.Methods32 Wistar female rats,randomly divided into 4 groups were shamly operated or ovariectomized,then all were fed with medicine for two months and then killed.The right tibiae were roasted and weighed to be dissolved by muriatic acid for testing Calcium,Magnesium,Phosphorus,and hydroxyproline.The left proximal and middle tibias were trimed and processed undercalcified sections for cancellous and cortical bone histomorphometric analysis.ResultsThe bone quality parameter such as Calcium,Magnesium,Phosphorus,hydroxyproline and bone weight obviously increased,the static and dynamic parameters of the cancellous bone histomorphometry similarly significantly increased,but the resorption parameters decreased markedly;meantime,the static and dynamic parameters of the cortical bone histomorphometry both had no significant change,while the bone resorption parameter in the endocortical region decreased obviously.ConclusionThe cancellous bone density of OVX rats can be evidently improved by increasing bone formation and inhibiting the endocortical bone resorption.The bone quality also be markedly improved by enhancing hydroxyproline secretion and osteoblast resorption to Calcium,Magnesium and Phosphorus and leading to new bone reconstruction.Therefore,the changes of the bone histomorphometry and bone quality parameter in ovariectomized rats have the same consistency.