Determination of Chlorogenic Acid,Caffeic Acid and Rutin in Different Medicinal Parts and Growth Stages of Solanum lyratum Thunb.

Objective To study contents of chlorogenic acid,caffeic acid and rutin in different medicinal parts and growth stage of Solanum lyratum Thunb.and to provide a theoretical basis for confirming an optimal recovery season.Methods The root,stem,leaf and fruit samples were collected from May to November.High performance liquid chromatography(HPLC) method was used.The Angilent Zorbax ODS C18 columm(4.6 mm×250 mm,5 μm) was used.The detection wavelength was 327 nm.The mobile phase consisted of acetonitrile-1% acetum,and the flow rate was 0.6 mL·min-1.The column temperature was set at 25 ℃.Results Chlorogenic acid in different medicinal parts of Solanum lyratum Thunb.was comparatively abundant,the distribution of chlorogenic acid content met the following rules: fruitupper part stemrootunder part stemleaf.The maximum values of chlorogenic acid at different medicinal parts were as follows: 2.583 mg·g-1 in the root at August,2.762 mg·g-1 in the under part stem at August,1.788 mg·g-1 in the upper part stem at september.The content of chlorogenic acid,caffeic acid and rutin in the leaf was comparatively abundant.The maximum value of caffeic acid in the leaf was 0.397 mg·g-1 at August.The maximum values of rutin and chlorogenic acid were 3.777 mg·g-1 and 8.169 mg·g-1 at November,respectively.Conclusion The contents of chlorogenic acid,caffeic acid and rutin change as Solanum lyratum Thunb.grows and develops.The active constituent content is most abundant in the leafiness Solanum lyratum Thunb.harvested at August or September.