Identification of High and Low Molecular Weight Glutenin Genes and 1B/1R Translocation in Sichuan Wheat Cultivars with Molecular Markers

The composition of high and low molecular weight glutenins and 1B/1Rtranslocation is key information on wheat quality breeding.Using a total of 25 DNA molecular markers,including 7markers specific to HMW-GS,17 to LMW-GS and 1to 1B/1Rtranslocation,105 Sichuan wheat cultivars released since 2000 were identified.The results showed that:(1)For HMW-GS,2cultivars contained Ax2*at Glu-A1 locus,with a frequency of 1.9%.At Glu-B1 locus,73cultivars had Bx7,26 carried Bx20,4possessed Bx17,45 had By8 and 30 carried By9,with a percentage of 69.5%,24.8%,3.8%,42.9% and 28.6%,respectively.At Glu-D1 locus,65 cultivars contained Dx5,with a frequency of 61.9%.(2)For LMW-GS,the genes frequencies of the Glu-A3 a,Glu-A3 b,Glu-A3 c,GluA3d,and Glu-A3 fat Glu-A3 locus were 1.9%,1.9%,60.0%,27.6% and 8.6%,respectively.At Glu-B3 locus,the frequencies of the Glu-B3 b,Glu-B3 d,Glu-B3 f,Glu-B3 gand Glu-B3 iwere 17.1%,9.5%,1.0%,71.4% and 1.0%,respectively.(3)Thirty-seven cultivars with 1B/1Rtranslocation were detected,with a frequency of 34.3%.(4)2(3.8%)and 15(14.3%)cultivars were found with a combination of 6or 5genes of Ax2*,Bx7,By8,Dx5,Glu-A3 d,Glu-B3 d,Glu-A3 b,Glu-B3 band non-1B/1Rtranslocation.These cultivars can be used as parents in wheat breeding for introducing genes with better quality potential related to gluten quality of Sichuan wheat cultivars.