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Effect of inhibition of extracellular-signal regulated protein kinase on As_2O_3-induced apoptosis in gastric cancer MGC-803 cells

Dan Li

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Abstract

OBJECTIVE:To explore the effect of inhibition of extracellular-signal regulated protein kinase on As2O3-induced apoptosis in gastric cancer MGC-803 cell.METHODS:The growth inhibition of tumor cells was determined by MTT method,and the ratios of apoptosis were determined by Wright-Giemsa and flow cytometric analysis.RESULTS:The MGC803 cell line was treated with the concentoations(5 and 10 μmol/L)of As2O3 for 24 h,and the apoptic ratios were(4.73±0.03)% and(11.93±0.06)% respectirely.After treated with As2O3 and PD98059,the apoptic ratios were augments to(11.65±0.09)% and(18.15±0.10)%,respectirely,P0.05.As2O3 inhibited cell growth of MGC-803 in a time and dose dependent manner.CONCLUSIONS:As2O3 induces MGC-803 cells apoptosis and the selective inhibition of ERK augmentes the effect of As2O3-induced apoptosis.ERK may have a relation to the apoptosis induced by As2O3 in MGC-803 cells.

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OBJECTIVE:To explore the effect of inhibition of extracellular-signal regulated protein kinase on As2O3-induced apoptosis in gastric cancer MGC-803 cell.METHODS:The growth inhibition of tumor cells was determined by MTT method,and the ratios of apoptosis were determined by Wright-Giemsa and flow cytometric analysis.RESULTS:The MGC803 cell line was treated with the concentoations(5 and 10 μmol/L)of As2O3 for 24 h,and the apoptic ratios were(4.73±0.03)% and(11.93±0.06)% respectirely.After treated with As2O3 and PD98059,the apoptic ratios were augments to(11.65±0.09)% and(18.15±0.10)%,respectirely,P0.05.As2O3 inhibited cell growth of MGC-803 in a time and dose dependent manner.CONCLUSIONS:As2O3 induces MGC-803 cells apoptosis and the selective inhibition of ERK augmentes the effect of As2O3-induced apoptosis.ERK may have a relation to the apoptosis induced by As2O3 in MGC-803 cells.

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Available abstract

OBJECTIVE:To explore the effect of inhibition of extracellular-signal regulated protein kinase on As2O3-induced apoptosis in gastric cancer MGC-803 cell.METHODS:The growth inhibition of tumor cells was determined by MTT method,and the ratios of apoptosis were determined by Wright-Giemsa and flow cytometric analysis.RESULTS:The MGC803 cell line was treated with the concentoations(5 and 10 μmol/L)of As2O3 for 24 h,and the apoptic ratios were(4.73±0.03)% and(11.93±0.06)% respectirely.After treated with As2O3 and PD98059,the apoptic ratios were augments to(11.65±0.09)% and(18.15±0.10)%,respectirely,P0.05.As2O3 inhibited cell growth of MGC-803 in a time and dose dependent manner.CONCLUSIONS:As2O3 induces MGC-803 cells apoptosis and the selective inhibition of ERK augmentes the effect of As2O3-induced apoptosis.ERK may have a relation to the apoptosis induced by As2O3 in MGC-803 cells.

Key concepts: Apoptosis, MAPK/ERK pathway, Extracellular, Kinase, Molecular biology, Growth inhibition, Cell growth, Cancer cell

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