Analysis on the Changes of Phytohormones During Flower Bud Development in Male-sterile Radish

The changes of the contents of endogenous phytohormones were measured by ELISA(enzymelinked immunosorbent assay)to analyse the dynamic changes of endogenous phytohormones in different development stages of buds between male sterility and fertile radish.The results showed that the change of the phytohormones contents was significant during the whole bud development stages in male fertile and sterile plants at the development stage when the sterile phenomenon begin to appear,the content of IAA in male sterile bud was higher 32.09% than that in the normal fertile bud;while ABA and JA content were 75.45% and 16.18% lower than that of CK,respectively.The contents of the other two phytohormones change little.The change of IAA/ABA,IAA/ZR,IAA/GA3,GA3/ABA were inconsistent,and the discrepancy of them was significant,IAA/ABA,IAA/ZR and GA3/ABA showed inbalance when the sterile phenomenon began to appear,and suggested that endogenous hormones may be related to male sterility tightly in radish.