Construction of transforming growth factor beta 1 antisense RNA recombinant adenovirus

Objective To construct transforming growth factor beta 1 (TGF-β) antisense RNA via recombinant adenovirus. Methods Partial cDNA of TGF-β1 was inserted into adenoviral vector pAdTrack-CMV and then was recombinated with packaging plasmid pAdEasy-1 in BJ5 183 bacteria. The adenovirus was generated in human 293 cells via homologous recomhination. A series of methods such as polymerase chain reaction (PCR) and fluorescence microscopy was employed to identify the generated recombinant adenovirus. Results Recombinant TGF-β1 antisense adenoviruses were constructed and the titer of virus was generally up to 2. 4 × 1 09 phaque forming units per milliliter (PFU /ml). Conclusion The recombinant adenovirus may be useful in the research and treatment for proliferative glomerulonephritis and sclerosing glomerulonephritis.