2003•Unpublished venueRequires access
Effects of Wortmannin Inhibiting Phosphatidylinositol-3 Kinase's Approach on K562 and NB4 Cell Proliferation
LI Zhengfa
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Abstract
To explore the effects of wortmannin inhibiting phosphatidylinositol-3 kinase's approach on K562 and NB4 cell proliferation and try to find out a new approach treating chronic myelogenous leukemia. Using wortmannin to restrain the PI-3K vitality in 0.25?μmol/L consistency and observing their effects on K562 cells and NB4 cells. The results showed that the growth inhibition rates of K562 cells were 41.33%,57.46%,65.85% respectively, NB4 cells were 26.29%, 5.51%, 2.10% respectively in 24?hours,48?hours,72?hours; that K562 and NB4 cells colony formation rates were 16.15% ,7.60%,5.90%,6.10% and colony formation inhibition rates were 52.94% and 3.39% . Wortmannin can inhibit K562 cells proliferation, but had no effect on NB4 cells proliferation. Conclusion: Wortmannin can inhibit the K562 cells proliferation by suppressing PI3K activity.