The Effect of Homocyeteine on Endothelial Function in the State of Calcium Ionophore

To study the state of calcium ionophore,the effect of homocyeteine on the activites of eNOS and of contents of NO in HUVEC.HUVECs were collected and cultured to the second passage.Then HUVECs were co-cultured with or without homocyeteine or A23187 for 24 hours.The NO,eNOS,MDA,SOD in culture medium were examined.Compared with control group,the activity of eNOS and the content of NO in medium in homocysteine group was significantly reduced but SOD and MDA was significantly increased(P0.05),In A23187 group,the activity of eNOS and the content of NO was increased significantly,MDA was decreased,SOD was increased.Compared with A23187 group,eNOS and NO was significantly reduced,MDA were increased in HCY+A23187 group,but SOD has no difference.Conclusion:homocysteine can decrease the content of NO and the activityof eNOS and increase the content of MDA and SOD in both base and activated state.The mechanisms may be concerned with oxidation stress that can inhibit the activity of eNOS and lower the NO bioavailability.