Comparative study on anti-tumor immune response of autologous CIK cells and allogeneic CIK cells

Objective To compare the anti-tumor immune response of autologous cytokine-induced killer(CIK) cells with that of allogeneic CIK cells and explore a more effective anti-tumor adoptive immunotherapy.Methods Peripheral monocytes were isolated from patients with renal carcinoma,lung cancer,or maxillary squamous cell carcinoma and their healthy adult children.Autologous CIK cells from patients were cultured alone as controls.The killing activity was detected by MTT assay;immunophenotype changes were analyzed by flow cytometry;the IL-12,IFN-γ and TNF-α levels of the cultured supernatants were detected by ELISA kits.Results The results showed that the proliferation capability of allogeneic CIK cells was significantly higher than that of autologous CIK cells.Compared with autologous CIK cells,allogeneic CIK cells significantly enhanced the anti-tumor activity and TNF-α secretion IFN-γ secretion,reduced IL-12 secretion,increased the ratio of CD3+CD56+ cells and CD3+CD8+ cells.Conclusion Allogeneic CIK cells have a stronger anti-tumor effect than autologous CIK cells.The results can provide experimental evidence for clinical application of CIK cells.